A Modified Hydroxyproline Assay Based on Hydrochloric Acid in Ehrlich's Solution Accurately Measures Tissue Collagen Content
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چکیده
منابع مشابه
A Modified Hydroxyproline Assay Based on Hydrochloric Acid in Ehrlich's Solution Accurately Measures Tissue Collagen Content.
Collagen quantification has long been relevant to biomedical research and clinical practice to characterize tissues and determine disease states. The hydroxyproline assay, while a broadly employed method of quantifying collagen, uses perchloric acid to dissolve Ehrlich's reagent. Since perchloric acid poses occupational safety hazards and high costs, in this study, a new hydroxyproline assay wa...
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A modified assay for the determination of hydroxyproline in tissue is presented. The modifications greatly reduce the time required for analysis of excised tissue as first introduced by Stegemann and Stalder [1]. These modifications include a change in the technique for tissue hydrolysis and a change in the preparation of the hydroxyproline oxidizing agent. The analysis utilizes the standard ad...
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Objective- To determine the effect of collagen type I on hydroxyproline content in Achilles tendon in dogs. Design- Experimental in vivo study. Animals- A total of 12 adult dogs. Procedure- Under general anesthesia and aseptic condition the dorsal surface of right Achilles tendon was exposed and after complete separation of connective tissue, it was splitted (striking 10 times) in full thic...
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Effectiveness of furfural as an organic inhibitor in 5 percent hydrochloric acid which is used in acid pickling of steel was evaluated. Lungmuir, Frumkin and Flory-Huggins isotherms [1, 2] were used to determine the parameters of effective adsorption, free energy and entropy of adsorption. Furfural indicated an inhibition less than 17 percent in concentrations of 0.001 mole per liter for a dura...
متن کاملInvestigation of Furfural as Corrosion Inhibitor in Hydrochloric Acid Solution
Effectiveness of furfural as an organic inhibitor in 5 percent hydrochloric acid which is used in acid pickling of steel was evaluated. Lungmuir, Frumkin and Flory-Huggins isotherms [1, 2] were used to determine the parameters of effective adsorption, free energy and entropy of adsorption. Furfural indicated an inhibition less than 17 percent in concentrations of 0.001 mole per liter for a dura...
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ژورنال
عنوان ژورنال: Tissue Engineering Part C: Methods
سال: 2017
ISSN: 1937-3384,1937-3392
DOI: 10.1089/ten.tec.2017.0018